How Cation Exchange Chromatography Works
This animation visualizes a charged resin column where negatively charged beads bind positively charged molecules flowing through in a mixed sample. It shows how molecules with stronger positive charge stick more tightly, while weakly charged or neutral molecules pass through first. An increasing salt gradient is shown displacing bound cations in order of charge strength, achieving separation. Useful for students studying biochemistry, analytical chemistry, or protein purification techniques.
16:9 · every frame verified for overlaps, spacing and edges before rendering
The prompt that made it
Show how cation exchange chromatography works
Make the next one in this series
- Show how anion exchange chromatography differs using positively charged resin beads
- Animate size exclusion chromatography separating molecules by molecular size
- Illustrate how pH gradients affect protein charge and elution in ion exchange columns
